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Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry (Record no. 1736)

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Personal name Pirone, Daniele
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9 (RLIN) 1645
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Title Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry
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Date of publication, distribution, etc. 2022-12.
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General note /pmc/articles/PMC7613862/
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General note /pubmed/36451849
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Summary, etc. Quantitative Phase Imaging (QPI) has gained popularity in bioimaging because it can avoid the need for cell staining, which in some cases is difficult or impossible. However, as a result, QPI does not provide labelling of various specific intracellular structures. Here we show a novel computational segmentation method based on statistical inference that makes it possible for QPI techniques to identify the cell nucleus. We demonstrate the approach with refractive index tomograms of stain-free cells reconstructed through the tomographic phase microscopy in flow cytometry mode. In particular, by means of numerical simulations and two cancer cell lines, we demonstrate that the nucleus can be accurately distinguished within the stain-free tomograms. We show that our experimental results are consistent with confocal fluorescence microscopy (FM) data and microfluidic cytofluorimeter outputs. This is a significant step towards extracting specific three-dimensional intracellular structures directly from the phase-contrast data in a typical flow cytometry configuration.
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Terms governing use and reproduction
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Terms governing use and reproduction https://www.springernature.com/gp/open-research/policies/accepted-manuscript-termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms
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Language note en
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Topical term or geographic name as entry element Article
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Genre/form data or focus term Text
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Personal name Lim, Joowon
Relator term author
9 (RLIN) 1646
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Personal name Merola, Francesco
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9 (RLIN) 1647
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Personal name Miccio, Lisa
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9 (RLIN) 1648
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Personal name Mugnano, Martina
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9 (RLIN) 1649
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Personal name Bianco, Vittorio
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9 (RLIN) 1650
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Personal name Cimmino, Flora
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9 (RLIN) 1651
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Personal name Visconte, Feliciano
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9 (RLIN) 1652
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Personal name Montella, Annalaura
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9 (RLIN) 1653
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Personal name Capasso, Mario
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9 (RLIN) 1654
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Personal name Iolascon, Achille
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9 (RLIN) 1655
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Personal name Memmolo, Pasquale
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9 (RLIN) 1656
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Personal name Psaltis, Demetri
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9 (RLIN) 1657
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Personal name Ferraro, Pietro
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9 (RLIN) 1658
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Note Nat Photonics
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Uniform Resource Identifier <a href="http://dx.doi.org/10.1038/s41566-022-01096-7">http://dx.doi.org/10.1038/s41566-022-01096-7</a>
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