JOM KITA KE POLITEKNIK

Nur77-Tempo mice reveal T cell steady state antigen recognition (Record no. 2002)

MARC details
042 ## - AUTHENTICATION CODE
Authentication code dc
100 10 - MAIN ENTRY--PERSONAL NAME
Personal name Elliot, Thomas A. E.
Relator term author
9 (RLIN) 2099
245 00 - TITLE STATEMENT
Title Nur77-Tempo mice reveal T cell steady state antigen recognition
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Date of publication, distribution, etc. 2022-12-22.
500 ## - GENERAL NOTE
General note /pmc/articles/PMC7614040/
500 ## - GENERAL NOTE
General note /pubmed/36704407
520 ## - SUMMARY, ETC.
Summary, etc. In lymphocytes, Nr4a gene expression is specifically regulated by antigen receptor signalling, making them ideal targets for use as distal T cell receptor (TCR) reporters. Nr4a3-Timer of cell kinetics and activity (Tocky) mice are a ground-breaking tool to report TCR-driven Nr4a3 expression using Fluorescent Timer protein (FT). FT undergoes a time-dependent shift in its emission spectrum following translation, allowing for the temporal reporting of transcriptional events. Our recent work suggested that Nr4a1/Nur77 may be a more sensitive gene to distal TCR signals compared to Nr4a3, so we, therefore, generated Nur77-Timer-rapidly-expressed-in-lymphocytes (Tempo) mice that express FT under the regulation of Nur77. We validated the ability of Nur77-Tempo mice to report TCR and B cell receptor signals and investigated the signals regulating Nur77-FT expression. We found that Nur77-FT was sensitive to low-strength TCR signals, and its brightness was graded in response to TCR signal strength. Nur77-FT detected positive selection signals in the thymus, and analysis of FT expression revealed that positive selection signals are often persistent in nature, with most thymic Treg expressing FT Blue. We found that active TCR signals in the spleen are low frequency, but CD69(+) lymphoid T cells are enriched for FT Blue(+) Red(+) T cells, suggesting frequent TCR signalling. In non-lymphoid tissue, we saw a dissociation of FT protein from CD69 expression, indicating that tissue residency is not associated with tonic TCR signals. Nur77-Tempo mice, therefore, combine the temporal dynamics from the Tocky innovation with increased sensitivity of Nr4a1 to lower TCR signal strengths.
540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE
Terms governing use and reproduction
540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE
Terms governing use and reproduction https://creativecommons.org/licenses/by/4.0/This work is licensed under a CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) International license.
546 ## - LANGUAGE NOTE
Language note en
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element Article
655 7# - INDEX TERM--GENRE/FORM
Genre/form data or focus term Text
Source of term local
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Jennings, Emma K.
Relator term author
9 (RLIN) 2100
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Lecky, David A. J.
Relator term author
9 (RLIN) 2101
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Rouvray, Sophie
Relator term author
9 (RLIN) 2102
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Mackie, Gillian M.
Relator term author
9 (RLIN) 2103
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Scarfe, Lisa
Relator term author
9 (RLIN) 2104
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Sheriff, Lozan
Relator term author
9 (RLIN) 2105
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Ono, Masahiro
Relator term author
9 (RLIN) 2106
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Maslowski, Kendle M.
Relator term author
9 (RLIN) 2107
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Bending, David
Relator term author
9 (RLIN) 2108
786 0# - DATA SOURCE ENTRY
Note Discov Immunol
856 41 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://dx.doi.org/10.1093/discim/kyac009">http://dx.doi.org/10.1093/discim/kyac009</a>
Public note Connect to this object online.

No items available.