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Bio-products from Serratia marcescens isolated from Ghanaian Anopheles gambiae reduce Plasmodium falciparum burden in vector mosquitoes (Record no. 2087)

MARC details
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Authentication code dc
100 10 - MAIN ENTRY--PERSONAL NAME
Personal name Akorli, Esinam Abla
Relator term author
9 (RLIN) 2774
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Title Bio-products from Serratia marcescens isolated from Ghanaian Anopheles gambiae reduce Plasmodium falciparum burden in vector mosquitoes
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Date of publication, distribution, etc. 2022-09-29.
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General note /pmc/articles/PMC7614139/
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General note /pubmed/36742111
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Summary, etc. Novel ideas for control of mosquito-borne disease include the use of bacterial symbionts to reduce transmission. Bacteria belonging to the family Enterobacteriaceae isolated from mosquito midgut have shown promise in limiting Plasmodium intensity in the Anopheles vector. However, the mechanism of interaction between bacteria and parasite remains unclear. This study aimed at screening bio-products of two bacteria candidates for their anti-Plasmodial effects on mosquito stages of P. falciparum. Enterobacter cloacae and Serratia marcescens were isolated from field-caught Anopheles gambiae s.l. Spent media from liquid cultures of these bacteria were filtered, lyophilized and dissolved in sterile phosphate buffered saline (PBS). The re-dissolved bacterial products were added to gametocytaemic blood meals and fed to An. gambiae mosquitoes via membrane feeders. Control groups were fed on infected blood with or without lyophilized LB medium. The effect of the products on the infection prevalence and intensity of P. falciparum in mosquitoes was assessed by dissecting mosquito midguts and counting oocysts 10-11 days post-infection. S. marcescens bio-products elicited significant reduction in the number of mosquitoes infected (P=4.02 x10(-5)) with P. falciparum and the oocyst intensity (P<2 x 10(-16)) than E. cloacae products (P>0.05 for both prevalence and intensity) compared to the control (lyophilized LB medium). These data support the use of bioproducts released by S. marcescens for malaria control based on transmission blocking in the vector.
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Terms governing use and reproduction
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Terms governing use and reproduction https://creativecommons.org/licenses/by/4.0/This work is licensed under a CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) International license.
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Language note en
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Topical term or geographic name as entry element Article
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Genre/form data or focus term Text
Source of term local
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Ubiaru, Prince Chigozirim
Relator term author
9 (RLIN) 2775
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Pradhan, Sabyasachi
Relator term author
9 (RLIN) 2776
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Akorli, Jewelna
Relator term author
9 (RLIN) 2777
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Ranford-Cartwright, Lisa
Relator term author
9 (RLIN) 2778
786 0# - DATA SOURCE ENTRY
Note Front Trop Dis
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Uniform Resource Identifier <a href="http://dx.doi.org/10.3389/fitd.2022.979615">http://dx.doi.org/10.3389/fitd.2022.979615</a>
Public note Connect to this object online.

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