Acute BAF perturbation causes immediate changes in chromatin accessibility (Record no. 2097)
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042 ## - AUTHENTICATION CODE | |
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Authentication code | dc |
100 10 - MAIN ENTRY--PERSONAL NAME | |
Personal name | Schick, Sandra |
Relator term | author |
9 (RLIN) | 2358 |
245 00 - TITLE STATEMENT | |
Title | Acute BAF perturbation causes immediate changes in chromatin accessibility |
260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
Date of publication, distribution, etc. | 2021-03-01. |
500 ## - GENERAL NOTE | |
General note | /pmc/articles/PMC7614082/ |
500 ## - GENERAL NOTE | |
General note | /pubmed/33558760 |
520 ## - SUMMARY, ETC. | |
Summary, etc. | Cancer-associated loss-of-function mutations in genes coding for subunits of the BRG1/BRM associated factor (BAF) chromatin remodeling complexes(1-8) often cause drastic chromatin accessibility changes, especially in important regulatory regions(9-19). However, it remains unknown how these changes are established over time (e.g. immediate consequences or long-term adaptations), and whether they are causative for intra-complex synthetic lethalities abrogating the formation or activity of BAF complexes(9,20-24). Here, we use the dTAG system to induce acute degradation of BAF subunits and show that chromatin alterations are established faster than the duration of one cell cycle. Using a pharmacological inhibitor and a chemical degrader of the BAF complex ATPase subunits(25,26), we show that maintaining genome accessibility requires constant ATP-dependent remodeling. Completely abolishing BAF complex function by acute degradation of a synthetic lethal subunit in a paralog-deficient background results in a near-complete loss of chromatin accessibility at BAF-controlled sites, especially at super-enhancers, providing a mechanism for intra-complex synthetic lethalities. |
540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE | |
Terms governing use and reproduction | |
540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE | |
Terms governing use and reproduction | http://www.nature.com/authors/editorial_policies/license.html#termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
546 ## - LANGUAGE NOTE | |
Language note | en |
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
Topical term or geographic name as entry element | Article |
655 7# - INDEX TERM--GENRE/FORM | |
Genre/form data or focus term | Text |
Source of term | local |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Grosche, Sarah |
Relator term | author |
9 (RLIN) | 2359 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Kohl, Katharina Eva |
Relator term | author |
9 (RLIN) | 2360 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Drpic, Danica |
Relator term | author |
9 (RLIN) | 2361 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Jaeger, Martin G. |
Relator term | author |
9 (RLIN) | 2362 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Marella, Nara C. |
Relator term | author |
9 (RLIN) | 2363 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Imrichova, Hana |
Relator term | author |
9 (RLIN) | 2364 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Lin, Jung-Ming G. |
Relator term | author |
9 (RLIN) | 2365 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Hofstätter, Gerald |
Relator term | author |
9 (RLIN) | 2366 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Schuster, Michael |
Relator term | author |
9 (RLIN) | 2367 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Rendeiro, André F. |
Relator term | author |
9 (RLIN) | 2368 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Koren, Anna |
Relator term | author |
9 (RLIN) | 2369 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Petronczki, Mark |
Relator term | author |
9 (RLIN) | 2370 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Bock, Christoph |
Relator term | author |
9 (RLIN) | 2371 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Müller, André C. |
Relator term | author |
9 (RLIN) | 2372 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Winter, Georg E. |
Relator term | author |
9 (RLIN) | 2373 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Kubicek, Stefan |
Relator term | author |
9 (RLIN) | 2374 |
786 0# - DATA SOURCE ENTRY | |
Note | Nat Genet |
856 41 - ELECTRONIC LOCATION AND ACCESS | |
Uniform Resource Identifier | <a href="http://dx.doi.org/10.1038/s41588-021-00777-3">http://dx.doi.org/10.1038/s41588-021-00777-3</a> |
Public note | Connect to this object online. |
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