JOM KITA KE POLITEKNIK

Application of loop mediated isothermal amplification (LAMP) assays for the detection of Onchocerca volvulus, Loa loa and Mansonella perstans in humans and vectors (Record no. 2271)

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Personal name Amambo, Glory Ngongeh
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9 (RLIN) 2401
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Title Application of loop mediated isothermal amplification (LAMP) assays for the detection of Onchocerca volvulus, Loa loa and Mansonella perstans in humans and vectors
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Date of publication, distribution, etc. 2023-01-09.
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General note /pmc/articles/PMC7614089/
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General note /pubmed/36684508
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Summary, etc. Conventional diagnosis of filarial infections is based on morphological identification of microfilariae using light microscopy and requires considerable expertise, is time-consuming, and can be subjective. Loop-mediated isothermal amplification (LAMP) has advantages over microscopy or PCR because of its operational simplicity, rapidity and versatility of readout options. LAMP assays represent a major step forward in improved filarial diagnostic tools suitable for low resource settings and field applicability. The study goal was to retrospectively evaluate the performance and suitability of the O-150, RF4, and Mp419 LAMP assays for diagnosing Onchocerca volvulus, Loa loa and Mansonella perstans infections, respectively, in humans and vectors under experimental and natural field conditions. Surveys were conducted in four health districts of Cameroon using skin snip and thick blood film methods to detect skin (O. volvulus) and blood (L. loa and M. perstans) dwelling microfilaria in humans. Engorged vectors (Simulium spp., Chrysops spp., and Culicoides spp.) were evaluated by LAMP. Dissected, wild-caught vectors were also analyzed. LAMP showed a prevalence of 40.4% (O. volvulus), 17.8% (L. loa) and 36.6% (M. perstans) versus 20.6% (O. volvulus), 17.4% (L. loa) and 33.8% (M. perstans) with microscopy. Simulium spp. were dissected for microscopy and pooled for LAMP. The O-150 LAMP assay infection rate was 4.3% versus 4.1% by microscopy. Chrysops spp. were dissected and analyzed individually in the LAMP assay. The RF4 LAMP assay infection rate was 23.5% versus 3.3% with microscopy. The RF4 LAMP assay also detected parasites in Chrysops spp. fed on low microfilaremic volunteers. The Mp419 LAMP assay infection rate was 0.2% for C. milnei and 0.04% for C. grahamii, while three other species were LAMP-negative. The sensitivity, species specificity, rapidity and ease of its use of these filarial LAMP assays, and validation of their performance in the field support use as alternatives to microscopy as diagnostic and surveillance tools in global health programs aimed to eliminate onchocerciasis.
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Terms governing use and reproduction
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Terms governing use and reproduction https://creativecommons.org/licenses/by/4.0/This work is licensed under a CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) International license.
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Terms governing use and reproduction https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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Personal name Innocentia, Ngong
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9 (RLIN) 2402
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Personal name Abong, Raphael Awah
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9 (RLIN) 2403
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Personal name Fombad, Fanny Fri
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9 (RLIN) 2404
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Personal name Njouendou, Abdel Jelil
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Personal name Nietcho, Franck
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9 (RLIN) 2406
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Personal name Ekanya, Relindis
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9 (RLIN) 2407
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Personal name Kien, Chi Anizette
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9 (RLIN) 2408
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Personal name Ebai, Rene
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9 (RLIN) 2409
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Personal name Lenz, Benjamin
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9 (RLIN) 2410
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Personal name Ritter, Manuel
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9 (RLIN) 2411
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Personal name Esum, Mathias Eyong
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9 (RLIN) 2412
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Personal name Deribe, Kebede
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9 (RLIN) 2413
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Personal name Cho, Jerome Fru
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9 (RLIN) 2414
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Personal name Beng, Amuam Andrew
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9 (RLIN) 2415
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Personal name Enyong, Peter Ivo
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9 (RLIN) 2416
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Personal name Li, Zhiru
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9 (RLIN) 2417
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Personal name Hübner, Marc P.
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9 (RLIN) 2418
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Personal name Pfarr, Kenneth
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9 (RLIN) 2419
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Personal name Hoerauf, Achim
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9 (RLIN) 2420
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Personal name Carlow, Clotilde
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9 (RLIN) 2421
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Personal name Wanji, Samuel
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9 (RLIN) 2422
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Note Front Trop Dis
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Uniform Resource Identifier <a href="http://dx.doi.org/10.3389/fitd.2022.1016176">http://dx.doi.org/10.3389/fitd.2022.1016176</a>
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