LC-MS/MS based detection of circulating proinsulin derived peptides in patients with altered pancreatic beta cell function (Record no. 513)
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042 ## - AUTHENTICATION CODE | |
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Authentication code | dc |
100 10 - MAIN ENTRY--PERSONAL NAME | |
Personal name | Foreman, Rachel E |
Relator term | author |
9 (RLIN) | 3116 |
245 00 - TITLE STATEMENT | |
Title | LC-MS/MS based detection of circulating proinsulin derived peptides in patients with altered pancreatic beta cell function |
260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
Date of publication, distribution, etc. | 2022-11-15. |
500 ## - GENERAL NOTE | |
General note | /pmc/articles/PMC7614196/ |
500 ## - GENERAL NOTE | |
General note | /pubmed/36242807 |
520 ## - SUMMARY, ETC. | |
Summary, etc. | Routine immunoassays for insulin and C-peptide have the potential to cross-react with partially processed proinsulin products, although in healthy patients these are present at such low levels that the interference is insignificant. Elevated concentrations of proinsulin and des-31,32 proinsulin arising from pathological conditions, or injected insulin analogues, however can cause significant assay interferences, complicating interpretation. Clinical diagnosis and management therefore sometimes require methods that can distinguish true insulin and C-peptide from partially processed proinsulin or injected insulin analogues. In this scenario, the high specificity of mass spectrometric analysis offers potential benefit for patient care. A high throughput targeted LC-MS/MS method was developed as a fit for purpose investigation of insulin, insulin analogues, C-peptide and proinsulin processing intermediates in plasma samples from different patient groups. Using calibration standards and bovine insulin as an internal standard, absolute concentrations of insulin and C-peptide were quantified across a nominal human plasma postprandial range and correlated strongly with immunoassay-based measurements. The ability to distinguish between insulin, insulin analogues and proinsulin intermediates in a single extraction is an improvement over existing immunological based techniques, offering the advantage of exact identification of the species being measured. The method promises to aid in the detection of circulating peptides which have previously been overlooked but may interfere with standard insulin and C-peptide immunoassays. |
540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE | |
Terms governing use and reproduction | |
540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE | |
Terms governing use and reproduction | https://creativecommons.org/licenses/by/4.0/This work is licensed under a CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) International license. |
546 ## - LANGUAGE NOTE | |
Language note | en |
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
Topical term or geographic name as entry element | Article |
655 7# - INDEX TERM--GENRE/FORM | |
Genre/form data or focus term | Text |
Source of term | local |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Meek, Claire L |
Relator term | author |
9 (RLIN) | 3117 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Roberts, Geoffrey P |
Relator term | author |
9 (RLIN) | 3118 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | George, Amy L |
Relator term | author |
9 (RLIN) | 3119 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Reimann, Frank |
Relator term | author |
9 (RLIN) | 3120 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Gribble, Fiona M |
Relator term | author |
9 (RLIN) | 3121 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Kay, Richard G |
Relator term | author |
9 (RLIN) | 3122 |
786 0# - DATA SOURCE ENTRY | |
Note | J Chromatogr B Analyt Technol Biomed Life Sci |
856 41 - ELECTRONIC LOCATION AND ACCESS | |
Uniform Resource Identifier | <a href="http://dx.doi.org/10.1016/j.jchromb.2022.123482">http://dx.doi.org/10.1016/j.jchromb.2022.123482</a> |
Public note | Connect to this object online. |
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