Evolutionary balance between foldability and functionality of a glucose transporter (Record no. 773)
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042 ## - AUTHENTICATION CODE | |
---|---|
Authentication code | dc |
100 10 - MAIN ENTRY--PERSONAL NAME | |
Personal name | Choi, Hyun-Kyu |
Relator term | author |
9 (RLIN) | 1112 |
245 00 - TITLE STATEMENT | |
Title | Evolutionary balance between foldability and functionality of a glucose transporter |
260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
Date of publication, distribution, etc. | 2022-07-01. |
500 ## - GENERAL NOTE | |
General note | /pmc/articles/PMC7612945/ |
500 ## - GENERAL NOTE | |
General note | /pubmed/35484435 |
520 ## - SUMMARY, ETC. | |
Summary, etc. | Despite advances in resolving structures of multi-pass membrane proteins, little is known about the native folding pathways of these complex structures. Using single-molecule magnetic tweezers, we here report a folding pathway of purified human glucose transporter 3 (GLUT3) reconstituted within synthetic lipid bilayers. The N-terminal major facilitator superfamily (MFS) fold strictly forms first, serving as structural templates for its C-terminal counterpart, in which polar residues comprising the conduit for glucose molecules present major folding challenges. The ER membrane protein complex facilitates insertion of these hydrophilic transmembrane helices, thrusting GLUT3's microstate sampling toward folded structures. Final assembly between the N- and C-terminal MFS folds depends on specific lipids that ease desolvation of lipid shells surrounding the domain interfaces. Sequence analysis suggests that this asymmetric folding propensity across the N-and C-terminal MFS folds prevails for metazoan sugar porters, revealing evolutionary conflicts between foldability and functionality faced by many multi-pass membrane proteins. |
540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE | |
Terms governing use and reproduction | |
546 ## - LANGUAGE NOTE | |
Language note | en |
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
Topical term or geographic name as entry element | Article |
655 7# - INDEX TERM--GENRE/FORM | |
Genre/form data or focus term | Text |
Source of term | local |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Kang, Hyunook |
Relator term | author |
9 (RLIN) | 1113 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Lee, Chanwoo |
Relator term | author |
9 (RLIN) | 1114 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Kim, Hyun Gyu |
Relator term | author |
9 (RLIN) | 1115 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Phillips, Ben P. |
Relator term | author |
9 (RLIN) | 1116 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Park, Soohyung |
Relator term | author |
9 (RLIN) | 1117 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Tumescheit, Charlotte |
Relator term | author |
9 (RLIN) | 1118 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Kim, Sang Ah |
Relator term | author |
9 (RLIN) | 1119 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Lee, Hansol |
Relator term | author |
9 (RLIN) | 1120 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Roh, Soung-Hun |
Relator term | author |
9 (RLIN) | 1121 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Hong, Heedeok |
Relator term | author |
9 (RLIN) | 1122 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Steinegger, Martin |
Relator term | author |
9 (RLIN) | 1123 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Im, Wonpil |
Relator term | author |
9 (RLIN) | 1124 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Miller, Elizabeth A. |
Relator term | author |
9 (RLIN) | 1125 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Choi, Hee-Jung |
Relator term | author |
9 (RLIN) | 1126 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Yoon, Tae-Young |
Relator term | author |
9 (RLIN) | 1127 |
786 0# - DATA SOURCE ENTRY | |
Note | Nat Chem Biol |
856 41 - ELECTRONIC LOCATION AND ACCESS | |
Uniform Resource Identifier | <a href="http://dx.doi.org/10.1038/s41589-022-01002-w">http://dx.doi.org/10.1038/s41589-022-01002-w</a> |
Public note | Connect to this object online. |
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