JOM KITA KE POLITEKNIK

Evolutionary balance between foldability and functionality of a glucose transporter (Record no. 773)

MARC details
042 ## - AUTHENTICATION CODE
Authentication code dc
100 10 - MAIN ENTRY--PERSONAL NAME
Personal name Choi, Hyun-Kyu
Relator term author
9 (RLIN) 1112
245 00 - TITLE STATEMENT
Title Evolutionary balance between foldability and functionality of a glucose transporter
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Date of publication, distribution, etc. 2022-07-01.
500 ## - GENERAL NOTE
General note /pmc/articles/PMC7612945/
500 ## - GENERAL NOTE
General note /pubmed/35484435
520 ## - SUMMARY, ETC.
Summary, etc. Despite advances in resolving structures of multi-pass membrane proteins, little is known about the native folding pathways of these complex structures. Using single-molecule magnetic tweezers, we here report a folding pathway of purified human glucose transporter 3 (GLUT3) reconstituted within synthetic lipid bilayers. The N-terminal major facilitator superfamily (MFS) fold strictly forms first, serving as structural templates for its C-terminal counterpart, in which polar residues comprising the conduit for glucose molecules present major folding challenges. The ER membrane protein complex facilitates insertion of these hydrophilic transmembrane helices, thrusting GLUT3's microstate sampling toward folded structures. Final assembly between the N- and C-terminal MFS folds depends on specific lipids that ease desolvation of lipid shells surrounding the domain interfaces. Sequence analysis suggests that this asymmetric folding propensity across the N-and C-terminal MFS folds prevails for metazoan sugar porters, revealing evolutionary conflicts between foldability and functionality faced by many multi-pass membrane proteins.
540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE
Terms governing use and reproduction
546 ## - LANGUAGE NOTE
Language note en
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element Article
655 7# - INDEX TERM--GENRE/FORM
Genre/form data or focus term Text
Source of term local
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Kang, Hyunook
Relator term author
9 (RLIN) 1113
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Lee, Chanwoo
Relator term author
9 (RLIN) 1114
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Kim, Hyun Gyu
Relator term author
9 (RLIN) 1115
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Phillips, Ben P.
Relator term author
9 (RLIN) 1116
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Park, Soohyung
Relator term author
9 (RLIN) 1117
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Tumescheit, Charlotte
Relator term author
9 (RLIN) 1118
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Kim, Sang Ah
Relator term author
9 (RLIN) 1119
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Lee, Hansol
Relator term author
9 (RLIN) 1120
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Roh, Soung-Hun
Relator term author
9 (RLIN) 1121
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Hong, Heedeok
Relator term author
9 (RLIN) 1122
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Steinegger, Martin
Relator term author
9 (RLIN) 1123
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Im, Wonpil
Relator term author
9 (RLIN) 1124
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Miller, Elizabeth A.
Relator term author
9 (RLIN) 1125
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Choi, Hee-Jung
Relator term author
9 (RLIN) 1126
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Yoon, Tae-Young
Relator term author
9 (RLIN) 1127
786 0# - DATA SOURCE ENTRY
Note Nat Chem Biol
856 41 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://dx.doi.org/10.1038/s41589-022-01002-w">http://dx.doi.org/10.1038/s41589-022-01002-w</a>
Public note Connect to this object online.

No items available.