Tension at intercellular junctions is necessary for accurate orientation of cell division in the epithelium plane (Record no. 916)
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042 ## - AUTHENTICATION CODE | |
---|---|
Authentication code | dc |
100 10 - MAIN ENTRY--PERSONAL NAME | |
Personal name | Lisica, Ana |
Relator term | author |
9 (RLIN) | 2432 |
245 00 - TITLE STATEMENT | |
Title | Tension at intercellular junctions is necessary for accurate orientation of cell division in the epithelium plane |
260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
Name of publisher, distributor, etc. | National Academy of Sciences, |
Date of publication, distribution, etc. | 2022-12-01. |
500 ## - GENERAL NOTE | |
General note | /pmc/articles/PMC7614093/ |
500 ## - GENERAL NOTE | |
General note | /pubmed/36454762 |
520 ## - SUMMARY, ETC. | |
Summary, etc. | The direction in which a cell divides is set by the orientation of its mitotic spindle and is important for determining cell fate, controlling tissue shape, and maintaining tissue architecture. Divisions parallel to the epithelial plane sustain tissue expansion. By contrast, divisions perpendicular to the plane promote tissue stratification and lead to the loss of epithelial cells from the tissue-an event that has been suggested to promote metastasis. Much is known about the molecular machinery involved in orienting the spindle, but less is known about the contribution of mechanical factors, such as tissue tension, in ensuring spindle orientation in the plane of the epithelium. This is important as epithelia are continuously subjected to mechanical stresses. To explore this further, we subjected suspended epithelial monolayers devoid of extracellular matrix to varying levels of tissue tension to study the orientation of cell divisions relative to the tissue plane. This analysis revealed that lowering tissue tension by compressing epithelial monolayers or by inhibiting myosin contractility increased the frequency of out-of-plane divisions. Reciprocally, increasing tissue tension by elevating cell contractility or by tissue stretching restored accurate in-plane cell divisions. Moreover, a characterization of the geometry of cells within these epithelia suggested that spindles can sense tissue tension through its impact on tension at subcellular surfaces, independently of their shape. Overall, these data suggest that accurate spindle orientation in the plane of the epithelium relies on a threshold level of tension at intercellular junctions. |
540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE | |
Terms governing use and reproduction | Copyright © 2022 the Author(s). Published by PNAS. |
540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE | |
Terms governing use and reproduction | https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
546 ## - LANGUAGE NOTE | |
Language note | en |
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
Topical term or geographic name as entry element | Biological Sciences |
655 7# - INDEX TERM--GENRE/FORM | |
Genre/form data or focus term | Text |
Source of term | local |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Fouchard, Jonathan |
Relator term | author |
9 (RLIN) | 2433 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Kelkar, Manasi |
Relator term | author |
9 (RLIN) | 2434 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Wyatt, Tom P. J. |
Relator term | author |
9 (RLIN) | 2435 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Duque, Julia |
Relator term | author |
9 (RLIN) | 2436 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Ndiaye, Anne-Betty |
Relator term | author |
9 (RLIN) | 2437 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Bonfanti, Alessandra |
Relator term | author |
9 (RLIN) | 2438 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Baum, Buzz |
Relator term | author |
9 (RLIN) | 2439 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Kabla, Alexandre J. |
Relator term | author |
9 (RLIN) | 2440 |
700 10 - ADDED ENTRY--PERSONAL NAME | |
Personal name | Charras, Guillaume T. |
Relator term | author |
9 (RLIN) | 2441 |
786 0# - DATA SOURCE ENTRY | |
Note | Proc Natl Acad Sci U S A |
856 41 - ELECTRONIC LOCATION AND ACCESS | |
Uniform Resource Identifier | <a href="http://dx.doi.org/10.1073/pnas.2201600119">http://dx.doi.org/10.1073/pnas.2201600119</a> |
Public note | Connect to this object online. |
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