JOM KITA KE POLITEKNIK

Mechanism of an intramembrane chaperone for multipass membrane proteins (Record no. 927)

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Personal name Smalinskaitė, Luka
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9 (RLIN) 2506
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Title Mechanism of an intramembrane chaperone for multipass membrane proteins
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Date of publication, distribution, etc. 2022-11-01.
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General note /pmc/articles/PMC7614104/
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General note /pubmed/36261528
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Summary, etc. Multipass membrane proteins play numerous roles in biology and include receptors, transporters, ion channels, and enzymes(1,2). How multipass proteins are co-translationally inserted and folded at the endoplasmic reticulum (ER) is not well understood(2). The prevailing model posits that each transmembrane domain (TMD) of a multipass protein successively passes into the lipid bilayer through a front-side lateral gate of the Sec61 protein translocation channel(3-9). The PAT complex, an intramembrane chaperone comprising Asterix and CCDC47, engages early TMDs of multipass proteins to promote their biogenesis by an unknown mechanism(10). Here, biochemical and structural analysis of intermediates during multipass protein biogenesis revealed that the nascent chain is not engaged with Sec61, which is occluded and latched closed by CCDC47. Instead, Asterix binds to and redirects the substrate behind Sec61, where the PAT complex contributes to a multipass translocon surrounding a semi-enclosed lipid-filled cavity(11). Detection of multiple TMDs in this cavity after their emergence from the ribosome suggests that multipass proteins insert and fold behind Sec61. Accordingly, biogenesis of several multipass proteins was unimpeded by inhibitors of the Sec61 lateral gate. These findings elucidate the mechanism of an intramembrane chaperone and suggest a new framework for multipass membrane protein biogenesis at the ER.
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Language note en
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Topical term or geographic name as entry element Article
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Personal name Kim, Min Kyung
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9 (RLIN) 2507
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Personal name Lewis, Aaron J. O.
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9 (RLIN) 2508
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Personal name Keenan, Robert J.
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9 (RLIN) 2509
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Personal name Hegde, Ramanujan S.
Relator term author
9 (RLIN) 2510
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Note Nature
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Uniform Resource Identifier <a href="http://dx.doi.org/10.1038/s41586-022-05336-2">http://dx.doi.org/10.1038/s41586-022-05336-2</a>
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