JOM KITA KE POLITEKNIK

Lewis x-Carrying O-glycans are Candidate Modulators for Conceptus Attachment in Pigs (Record no. 998)

MARC details
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Authentication code dc
100 10 - MAIN ENTRY--PERSONAL NAME
Personal name Han, Kun
Relator term author
9 (RLIN) 3061
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Title Lewis x-Carrying O-glycans are Candidate Modulators for Conceptus Attachment in Pigs
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Date of publication, distribution, etc. 2022-11-19.
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General note /pmc/articles/PMC7614189/
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General note /pubmed/36401880
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Summary, etc. Successful attachment of conceptus to the uterine luminal epithelium (LE) is crucial for establishing a functional placenta in pigs. However, the underlying mechanisms are yet to be elucidated. The uterine LE-conceptus interface is enriched in various glycoconjugates essential to implantation. Using MALDI-MS profiling, we identified for the first time the O-glycan repertoire in pig endometrium during the conceptus attachment stage. The expression pattern of blood group A, O(H), Lewis x, y, a, b (Le(x), Le(y), Le(a), and Le(b)), the sialylated and sulfated Le(x) antigens in the uterine LE-conceptus interface was assessed using immunofluorescence assays. Notably, the Le(x)-carrying O-glycans exhibited a temporal-spatial expression pattern. They were absent in the endometrium on estrous cycle days but strongly and spatially presented in the conceptus and uterine LE to which the conceptus apposes during the early conceptus attachment stage. In addition, Le(x)-carrying O-glycans were co-localized with secreted phosphoprotein 1 (SPP1), a well-characterized factor that plays a role in promoting conceptus attachment through interacting with integrin αVβ3 and integrin αVβ6. Meanwhile, the immunoprecipitation assays revealed an interaction between the Le(x)-carrying O-glycans and SPP1, integrin αV, and integrin β6. Furthermore, we provided evidence that the β1,4-galactosyltransferase 1 (B4GALT1) gene is a potential regulator for Le(x) antigen expression in the uterine LE-conceptus interface during the early conceptus attachment stage. In conclusion, our findings show that Le(x)-carrying O-glycans, presumably dependent on B4GALT1 gene expression, might modulate conceptus attachment by interacting with the SPP1-integrin receptor complex in pigs.
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Terms governing use and reproduction
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Terms governing use and reproduction https://creativecommons.org/licenses/by/4.0/This work is licensed under a CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) International license.
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Language note en
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Topical term or geographic name as entry element Article
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Genre/form data or focus term Text
Source of term local
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Yue, Yulu
Relator term author
9 (RLIN) 3062
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Personal name Wang, Weiwei
Relator term author
9 (RLIN) 3063
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Wang, Feiyu
Relator term author
9 (RLIN) 3064
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Personal name Chai, Wengang
Relator term author
9 (RLIN) 3065
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Personal name Zhao, Shuhong
Relator term author
9 (RLIN) 3066
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Yu, Mei
Relator term author
9 (RLIN) 3067
786 0# - DATA SOURCE ENTRY
Note Biol Reprod
856 41 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://dx.doi.org/10.1093/biolre/ioac204">http://dx.doi.org/10.1093/biolre/ioac204</a>
Public note Connect to this object online.

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