TY  - BOOK
AU  - Rodriguez-Rodriguez,Noe
AU  - Clark,Paula A.
AU  - Gogoi,Mayuri
AU  - Ferreira,Ana C.F.
AU  - Kerscher,Bernhard
AU  - Crisp,Alastair
AU  - Jolin,Helen E.
AU  - Murphy,Jane E.
AU  - Sivasubramaniam,Meera
AU  - Pedro,Luisa
AU  - Walker,Jennifer A.
AU  - Heycock,Morgan W.D.
AU  - Shields,Jacqueline D.
AU  - Barlow,Jillian L.
AU  - McKenzie,Andrew N.J.
TI  - Identification of aceNKPs, a committed common progenitor population of the ILC1 and NK cell continuum
PY  - 2022///-11-29
PB  - National Academy of Sciences, 
KW  - Text
KW  - local
N1  - /pmc/articles/PMC7614094; /pubmed/36442116
N2  - The development of innate lymphoid cell (ILC) transcription factor reporter mice has shown a previously unexpected complexity in ILC hematopoiesis. Using novel polychromic mice to achieve higher phenotypic resolution, we have characterized bone marrow progenitors that are committed to the group 1 ILC lineage. These common ILC1/NK cell progenitors (ILC1/NKP), which we call "aceNKPs", are defined as lineage(-)Id2(+)IL-7Rα(+)CD25(-)α4β7(-)NKG2A/C/E(+)Bcl11b(-). In vitro, aceNKPs differentiate into group 1 ILCs, including NK-like cells that express Eomes without the requirement for IL-15, and produce IFN-γ and perforin upon IL-15 stimulation. Following reconstitution of Rag2(-/-)Il2rg(-/-) hosts, aceNKPs give rise to a spectrum of mature ILC1/NK cells (regardless of their tissue location) that cannot be clearly segregated into the traditional ILC1 and NK subsets, suggesting that group 1 ILCs constitute a dynamic continuum of ILCs that can develop from a common progenitor. In addition, aceNKP-derived ILC1/NK cells effectively ameliorate tumor burden in a model of lung metastasis, where they acquired a cytotoxic NK cell phenotype. Our results identify the primary ILC1/NK progenitor that lacks ILC2 or ILC3 potential and is strictly committed to ILC1/NK cell production irrespective of tissue homing
UR  - http://dx.doi.org/10.1073/pnas.2203454119
ER  -