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042 _adc
100 1 0 _aSingh, Archna
_eauthor
_91710
700 1 0 _aBaalasubramanian, Sivasankar
_eauthor
_91711
700 1 0 _aKalaivani, Mani
_eauthor
_91712
700 1 0 _aKapoor, Ridhima
_eauthor
_91713
700 1 0 _aBhagwat, Ketaki
_eauthor
_91714
700 1 0 _aGhosh-Jerath, Suparna
_eauthor
_91715
245 0 0 _aStandardisation and application of a novel multiplex assay for estimating micronutrient status and inflammatory markers in women of Sauria Paharia and Santhal tribes of Jharkhand
260 _c2022-02-04.
500 _a/pmc/articles/PMC7613878/
500 _a/pubmed/35115060
520 _aThis study aimed to document the method standardisation and assessment of micronutrient and inflammatory markers in women from indigenous tribal communities of Jharkhand using a low-volume, high-throughput assay. This cross-sectional study was done among women of the reproductive age group from Sauria Paharia and Santhal tribal households (HH) in selected villages. Capillary blood samples were collected from the women during a HH survey to estimate ferritin, soluble transferrin receptor, retinol binding protein 4 and inflammatory biomarkers, C-reactive protein (CRP) and α-1-acid glycoprotein (AGP) using a multiplex assay. Vitamin D and Hb were estimated using an LC-MS technique and cyanmethaemoglobin method, respectively. A multiplex Luminex-based method was developed and standardised. The assay was used to estimate biomarkers in samples from 413 women (178 and 235 from Sauria Paharia and Santhal tribes, respectively). Over 51 % of women had raised CRP or AGP levels. Fe status was significantly better in Sauria Paharia compared with the Santhal women. Anaemia prevalence was 72 % among Santhal women. The proportion of women with Fe deficiency increased after adjusting for inflammation. The overall prevalence of vitamin A deficiency and insufficiency was 25 and 34 %, respectively, with similar prevalence in both tribes. All Santhal women had sufficient vitamin D levels, while 25 and 20 % of Sauria Paharia women had insufficient and deficient vitamin D levels, respectively. Our low-volume, high-throughput multiplex assays may provide a feasible approach for assessing nutritional biomarkers in nutritionally vulnerable hard-to-reach communities.
540 _a
546 _aen
690 _aArticle
655 7 _aText
_2local
786 0 _nBr J Nutr
856 4 1 _uhttp://dx.doi.org/10.1017/S0007114522000320
_zConnect to this object online.
999 _c1748
_d1748