| 000 | 03048 am a22004093u 4500 | ||
|---|---|---|---|
| 042 | _adc | ||
| 100 | 1 | 0 |
_aLin, Xionghui _eauthor _91154 |
| 700 | 1 | 0 |
_aSwedlund, Benjamin _eauthor _91155 |
| 700 | 1 | 0 |
_aTon, Mai-Linh N. _eauthor _91156 |
| 700 | 1 | 0 |
_aGhazanfar, Shila _eauthor _91157 |
| 700 | 1 | 0 |
_aGuibentif, Carolina _eauthor _91158 |
| 700 | 1 | 0 |
_aPaulissen, Catherine _eauthor _91159 |
| 700 | 1 | 0 |
_aBaudelet, Elodie _eauthor _91160 |
| 700 | 1 | 0 |
_aPlaindoux, Elise _eauthor _91161 |
| 700 | 1 | 0 |
_aAchouri, Younes _eauthor _91162 |
| 700 | 1 | 0 |
_aCalonne, Emilie _eauthor _91163 |
| 700 | 1 | 0 |
_aDubois, Christine _eauthor _91164 |
| 700 | 1 | 0 |
_aMansfield, William _eauthor _91165 |
| 700 | 1 | 0 |
_aZaffran, Stéphane _eauthor _91166 |
| 700 | 1 | 0 |
_aMarioni, John C. _eauthor _91167 |
| 700 | 1 | 0 |
_aFuks, Francois _eauthor _91168 |
| 700 | 1 | 0 |
_aGöttgens, Berthold _eauthor _91169 |
| 700 | 1 | 0 |
_aLescroart, Fabienne _eauthor _91170 |
| 700 | 1 | 0 |
_aBlanpain, Cédric _eauthor _91171 |
| 245 | 0 | 0 | _aMesp1 controls the chromatin and enhancer landscapes essential for spatiotemporal patterning of early cardiovascular progenitors |
| 260 | _c2022-07-01. | ||
| 500 | _a/pmc/articles/PMC7613098/ | ||
| 500 | _a/pubmed/35817961 | ||
| 520 | _aThe mammalian heart arises from various populations of Mesp1-expressing cardiovascular progenitors (CPs) that are specified during the early stages of gastrulation. Mesp1 is a transcription factor (TF) that acts as a master regulator of CP specification and differentiation. However, how Mesp1 regulates the chromatin landscape of nascent mesodermal cells to define the temporal and spatial patterning of the distinct populations of CPs remains unknown. Here, by combining ChIP-seq, RNA-seq and ATAC-seq during mouse pluripotent stem cell differentiation, we defined the dynamic remodelling of the chromatin landscape mediated by Mesp1. We identified different enhancers that are temporally regulated to erase the pluripotent state and specify the pools of CPs that mediate heart development. We identified Zic2 and Zic3 as essential cofactors that act with Mesp1 to regulate its TF activity at key mesodermal enhancers, thereby regulating the chromatin remodelling and gene expression associated with the specification of the different populations of CPs in vivo. Our study identifies the dynamics of the chromatin landscape and enhancer remodelling associated with temporal patterning of early mesodermal cells into the distinct populations of CPs that mediate heart development. | ||
| 540 | _a | ||
| 540 | _ahttps://www.springernature.com/gp/open-research/policies/accepted-manuscript-termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms. | ||
| 546 | _aen | ||
| 690 | _aArticle | ||
| 655 | 7 |
_aText _2local |
|
| 786 | 0 | _nNat Cell Biol | |
| 856 | 4 | 1 |
_uhttp://dx.doi.org/10.1038/s41556-022-00947-3 _zConnect to this object online. |
| 999 |
_c1850 _d1850 |
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