000			01508 am a22002533u 4500
042			_adc
100	1	0	_aLi, Weihan _eauthor _91716
700	1	0	_aMaekiniemi, Anna _eauthor _91717
700	1	0	_aSato, Hanae _eauthor _91718
700	1	0	_aOsman, Christof _eauthor _91719
700	1	0	_aSinger, Robert H. _eauthor _91720
245	0	0	_aAn improved imaging system that corrects MS2-induced RNA destabilization
260			_c2022-12.
500			_a/pmc/articles/PMC7613886/
500			_a/pubmed/36357695
520			_aThe MS2-MCP imaging system is widely used to study mRNA spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by targeting them to the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.
540			_a
540			_ahttps://www.springernature.com/gp/open-research/policies/accepted-manuscript-termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms
546			_aen
690			_aArticle
655	7		_aText _2local
786	0		_nNat Methods
856	4	1	_uhttp://dx.doi.org/10.1038/s41592-022-01658-1 _zConnect to this object online.
999			_c1868 _d1868