000 02544 am a22002533u 4500
042 _adc
100 1 0 _aAkorli, Esinam Abla
_eauthor
_92774
700 1 0 _aUbiaru, Prince Chigozirim
_eauthor
_92775
700 1 0 _aPradhan, Sabyasachi
_eauthor
_92776
700 1 0 _aAkorli, Jewelna
_eauthor
_92777
700 1 0 _aRanford-Cartwright, Lisa
_eauthor
_92778
245 0 0 _aBio-products from Serratia marcescens isolated from Ghanaian Anopheles gambiae reduce Plasmodium falciparum burden in vector mosquitoes
260 _c2022-09-29.
500 _a/pmc/articles/PMC7614139/
500 _a/pubmed/36742111
520 _aNovel ideas for control of mosquito-borne disease include the use of bacterial symbionts to reduce transmission. Bacteria belonging to the family Enterobacteriaceae isolated from mosquito midgut have shown promise in limiting Plasmodium intensity in the Anopheles vector. However, the mechanism of interaction between bacteria and parasite remains unclear. This study aimed at screening bio-products of two bacteria candidates for their anti-Plasmodial effects on mosquito stages of P. falciparum. Enterobacter cloacae and Serratia marcescens were isolated from field-caught Anopheles gambiae s.l. Spent media from liquid cultures of these bacteria were filtered, lyophilized and dissolved in sterile phosphate buffered saline (PBS). The re-dissolved bacterial products were added to gametocytaemic blood meals and fed to An. gambiae mosquitoes via membrane feeders. Control groups were fed on infected blood with or without lyophilized LB medium. The effect of the products on the infection prevalence and intensity of P. falciparum in mosquitoes was assessed by dissecting mosquito midguts and counting oocysts 10-11 days post-infection. S. marcescens bio-products elicited significant reduction in the number of mosquitoes infected (P=4.02 x10(-5)) with P. falciparum and the oocyst intensity (P<2 x 10(-16)) than E. cloacae products (P>0.05 for both prevalence and intensity) compared to the control (lyophilized LB medium). These data support the use of bioproducts released by S. marcescens for malaria control based on transmission blocking in the vector.
540 _a
540 _ahttps://creativecommons.org/licenses/by/4.0/This work is licensed under a CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) International license.
546 _aen
690 _aArticle
655 7 _aText
_2local
786 0 _nFront Trop Dis
856 4 1 _uhttp://dx.doi.org/10.3389/fitd.2022.979615
_zConnect to this object online.
999 _c2087
_d2087