| 000 | 02753 am a22003013u 4500 | ||
|---|---|---|---|
| 042 | _adc | ||
| 100 | 1 | 0 |
_aArmstrong, James P. K. _eauthor _92288 |
| 700 | 1 | 0 |
_aPchelintseva, Ekaterina _eauthor _92289 |
| 700 | 1 | 0 |
_aTreumuth, Sirli _eauthor _92290 |
| 700 | 1 | 0 |
_aCampanella, Cristiana _eauthor _92291 |
| 700 | 1 | 0 |
_aMeinert, Christoph _eauthor _92292 |
| 700 | 1 | 0 |
_aKlein, Travis J. _eauthor _92293 |
| 700 | 1 | 0 |
_aHutmacher, Dietmar W. _eauthor _92294 |
| 700 | 1 | 0 |
_aDrinkwater, Bruce W. _eauthor _92295 |
| 700 | 1 | 0 |
_aStevens, Molly M. _eauthor |
| 245 | 0 | 0 | _aTissue Engineering Cartilage with Deep Zone Cytoarchitecture by High-Resolution Acoustic Cell Patterning |
| 260 | _c2022-07-11. | ||
| 500 | _a/pmc/articles/PMC7614068/ | ||
| 500 | _a/pubmed/35815530 | ||
| 520 | _aThe ultimate objective of tissue engineering is to fabricate artificial living constructs with a structural organization and function that faithfully resembles their native tissue counterparts. For example, the deep zone of articular cartilage possesses a distinctive anisotropic architecture with chondrocytes organized in aligned arrays ≈1-2 cells wide, features that are oriented parallel to surrounding extracellular matrix fibers and orthogonal to the underlying subchondral bone. Although there are major advances in fabricating custom tissue architectures, it remains a significant technical challenge to precisely recreate such fine cellular features in vitro. Here, it is shown that ultrasound standing waves can be used to remotely organize living chondrocytes into high-resolution anisotropic arrays, distributed throughout the full volume of agarose hydrogels. It is demonstrated that this cytoarchitecture is maintained throughout a five-week course of in vitro tissue engineering, producing hyaline cartilage with cellular and extracellular matrix organization analogous to the deep zone of native articular cartilage. It is anticipated that this acoustic cell patterning method will provide unprecedented opportunities to interrogate in vitro the contribution of chondrocyte organization to the development of aligned extracellular matrix fibers, and ultimately, the design of new mechanically anisotropic tissue grafts for articular cartilage regeneration. | ||
| 540 | _a | ||
| 540 | _ahttps://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/) | ||
| 546 | _aen | ||
| 690 | _aArticle | ||
| 655 | 7 |
_aText _2local |
|
| 786 | 0 | _nAdv Healthc Mater | |
| 856 | 4 | 1 |
_uhttp://dx.doi.org/10.1002/adhm.202200481 _zConnect to this object online. |
| 999 |
_c2229 _d2229 |
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