| 000 | 02668 am a22002533u 4500 | ||
|---|---|---|---|
| 042 | _adc | ||
| 100 | 1 | 0 |
_aTichy, Harald _eauthor _93141 |
| 700 | 1 | 0 |
_aZeiner, Reinhard _eauthor _93142 |
| 700 | 1 | 0 |
_aTraunmüller, Peter _eauthor _93143 |
| 700 | 1 | 0 |
_aMartzok, Alexander _eauthor _93144 |
| 700 | 1 | 0 |
_aHellwig, Maria _eauthor _93145 |
| 245 | 0 | 0 | _aDeveloping and testing of an air dilution flow olfactometer with known rates of concentration change |
| 260 | _c2020-07-15. | ||
| 500 | _a/pmc/articles/PMC7614200/ | ||
| 500 | _a/pubmed/32446941 | ||
| 520 | _aBACKGROUND: Concentration is a variable aspect of an odor signal and determines the operation range of olfactory receptor neurons (ORNs). A concentration increase is perceived as an odor stimulus. The role that the rate of concentration increase plays thereby has been studied with electrophysiological techniques in ORNs of the cockroach. A key prerequisite for these studies was the development of an air dilution flow olfactometer that allowed testing the same change in concentration at various rates. NEW METHOD: The rate of concentration change was controlled and varied by changing the mixing ratio of odor-saturated and clean air by means of proportional valves. Their input voltages were phase shifted by 180° to hold the mixed air at a particular constant volume flow rate. RESULTS: Using this stimulation technique, we identified, in a morphologically distinct sensillum on the cockroach's antenna, antagonistically responding ON and OFF ORNs which display a high sensitivity for slow changes in odor concentration. COMPARISON WITH EXISTING METHODS: The olfactometer is unique because it enables delivering slowly oscillating concentration changes. By varying the oscillation period, the individual effects of the instantaneous odor concentration and its rate of change on the ORNs' responses can be determined. CONCLUSIONS: The olfactometer provides a new experimental approach in the study of odor coding and opens the door for improved comparative studies on olfactory systems. It would be important to gain insight into the ORNs' ability to detect the rate of concentration change in other insects that use odors for orientation in different contexts. | ||
| 540 | _a | ||
| 540 | _ahttps://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/BY-NC-ND/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). | ||
| 546 | _aen | ||
| 690 | _aArticle | ||
| 655 | 7 |
_aText _2local |
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| 786 | 0 | _nJ Neurosci Methods | |
| 856 | 4 | 1 |
_uhttp://dx.doi.org/10.1016/j.jneumeth.2020.108794 _zConnect to this object online. |
| 999 |
_c2236 _d2236 |
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