| 000 | 02665 am a22003493u 4500 | ||
|---|---|---|---|
| 042 | _adc | ||
| 100 | 1 | 0 |
_aSonam, Surabhi _eauthor _92319 |
| 700 | 1 | 0 |
_aBalasubramaniam, Lakshmi _eauthor _92320 |
| 700 | 1 | 0 |
_aLin, Shao-Zhen _eauthor _92321 |
| 700 | 1 | 0 |
_aIvan, Ying Ming Yow _eauthor _92322 |
| 700 | 1 | 0 |
_aJaumà, Irina Pi _eauthor _92323 |
| 700 | 1 | 0 |
_aJebane, Cecile _eauthor _92324 |
| 700 | 1 | 0 |
_aKarnat, Marc _eauthor _92325 |
| 700 | 1 | 0 |
_aToyama, Yusuke _eauthor _92326 |
| 700 | 1 | 0 |
_aMarcq, Philippe _eauthor _92327 |
| 700 | 1 | 0 |
_aProst, Jacques _eauthor _92328 |
| 700 | 1 | 0 |
_aMège, René-Marc _eauthor _92329 |
| 700 | 1 | 0 |
_aRupprecht, Jean-François _eauthor _92330 |
| 700 | 1 | 0 |
_aLadoux, Benoît _eauthor _92331 |
| 245 | 0 | 0 | _aMechanical stress driven by rigidity sensing governs epithelial stability |
| 260 | _c2023-01. | ||
| 500 | _a/pmc/articles/PMC7614076/ | ||
| 500 | _a/pubmed/36686215 | ||
| 520 | _aEpithelia act as a barrier against environmental stress and abrasion and in vivo they are continuously exposed to environments of various mechanical properties. The impact of this environment on epithelial integrity remains elusive. By culturing epithelial cells on 2D hydrogels, we observe a loss of epithelial monolayer integrity through spontaneous hole formation when grown on soft substrates. Substrate stiffness triggers an unanticipated mechanical switch of epithelial monolayers from tensile on soft to compressive on stiff substrates. Through active nematic modelling, we find that spontaneous half-integer defect formation underpinning large isotropic stress fluctuations initiate hole opening events. Our data show that monolayer rupture due to high tensile stress is promoted by the weakening of cell-cell junctions that could be induced by cell division events or local cellular stretching. Our results show that substrate stiffness provides feedback on monolayer mechanical state and that topological defects can trigger stochastic mechanical failure, with potential application towards a mechanistic understanding of compromised epithelial integrity during immune response and morphogenesis. | ||
| 540 | _a | ||
| 540 | _ahttps://www.springernature.com/gp/open-research/policies/accepted-manuscript-termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms | ||
| 546 | _aen | ||
| 690 | _aArticle | ||
| 655 | 7 |
_aText _2local |
|
| 786 | 0 | _nNat Phys | |
| 856 | 4 | 1 |
_uhttp://dx.doi.org/10.1038/s41567-022-01826-2 _zConnect to this object online. |
| 999 |
_c2243 _d2243 |
||