000 | 03013 am a22003133u 4500 | ||
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042 | _adc | ||
100 | 1 | 0 |
_aLisica, Ana _eauthor _92432 |
700 | 1 | 0 |
_aFouchard, Jonathan _eauthor _92433 |
700 | 1 | 0 |
_aKelkar, Manasi _eauthor _92434 |
700 | 1 | 0 |
_aWyatt, Tom P. J. _eauthor _92435 |
700 | 1 | 0 |
_aDuque, Julia _eauthor _92436 |
700 | 1 | 0 |
_aNdiaye, Anne-Betty _eauthor _92437 |
700 | 1 | 0 |
_aBonfanti, Alessandra _eauthor _92438 |
700 | 1 | 0 |
_aBaum, Buzz _eauthor _92439 |
700 | 1 | 0 |
_aKabla, Alexandre J. _eauthor _92440 |
700 | 1 | 0 |
_aCharras, Guillaume T. _eauthor _92441 |
245 | 0 | 0 | _aTension at intercellular junctions is necessary for accurate orientation of cell division in the epithelium plane |
260 |
_bNational Academy of Sciences, _c2022-12-01. |
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500 | _a/pmc/articles/PMC7614093/ | ||
500 | _a/pubmed/36454762 | ||
520 | _aThe direction in which a cell divides is set by the orientation of its mitotic spindle and is important for determining cell fate, controlling tissue shape, and maintaining tissue architecture. Divisions parallel to the epithelial plane sustain tissue expansion. By contrast, divisions perpendicular to the plane promote tissue stratification and lead to the loss of epithelial cells from the tissue-an event that has been suggested to promote metastasis. Much is known about the molecular machinery involved in orienting the spindle, but less is known about the contribution of mechanical factors, such as tissue tension, in ensuring spindle orientation in the plane of the epithelium. This is important as epithelia are continuously subjected to mechanical stresses. To explore this further, we subjected suspended epithelial monolayers devoid of extracellular matrix to varying levels of tissue tension to study the orientation of cell divisions relative to the tissue plane. This analysis revealed that lowering tissue tension by compressing epithelial monolayers or by inhibiting myosin contractility increased the frequency of out-of-plane divisions. Reciprocally, increasing tissue tension by elevating cell contractility or by tissue stretching restored accurate in-plane cell divisions. Moreover, a characterization of the geometry of cells within these epithelia suggested that spindles can sense tissue tension through its impact on tension at subcellular surfaces, independently of their shape. Overall, these data suggest that accurate spindle orientation in the plane of the epithelium relies on a threshold level of tension at intercellular junctions. | ||
540 | _aCopyright © 2022 the Author(s). Published by PNAS. | ||
540 | _ahttps://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . | ||
546 | _aen | ||
690 |
_aBiological Sciences _92442 |
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655 | 7 |
_aText _2local |
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786 | 0 | _nProc Natl Acad Sci U S A | |
856 | 4 | 1 |
_uhttp://dx.doi.org/10.1073/pnas.2201600119 _zConnect to this object online. |
999 |
_c425 _d425 |