000			03013 am a22003133u 4500
042			_adc
100	1	0	_aLisica, Ana _eauthor _92432
700	1	0	_aFouchard, Jonathan _eauthor _92433
700	1	0	_aKelkar, Manasi _eauthor _92434
700	1	0	_aWyatt, Tom P. J. _eauthor _92435
700	1	0	_aDuque, Julia _eauthor _92436
700	1	0	_aNdiaye, Anne-Betty _eauthor _92437
700	1	0	_aBonfanti, Alessandra _eauthor _92438
700	1	0	_aBaum, Buzz _eauthor _92439
700	1	0	_aKabla, Alexandre J. _eauthor _92440
700	1	0	_aCharras, Guillaume T. _eauthor _92441
245	0	0	_aTension at intercellular junctions is necessary for accurate orientation of cell division in the epithelium plane
260			_bNational Academy of Sciences, _c2022-12-01.
500			_a/pmc/articles/PMC7614093/
500			_a/pubmed/36454762
520			_aThe direction in which a cell divides is set by the orientation of its mitotic spindle and is important for determining cell fate, controlling tissue shape, and maintaining tissue architecture. Divisions parallel to the epithelial plane sustain tissue expansion. By contrast, divisions perpendicular to the plane promote tissue stratification and lead to the loss of epithelial cells from the tissue-an event that has been suggested to promote metastasis. Much is known about the molecular machinery involved in orienting the spindle, but less is known about the contribution of mechanical factors, such as tissue tension, in ensuring spindle orientation in the plane of the epithelium. This is important as epithelia are continuously subjected to mechanical stresses. To explore this further, we subjected suspended epithelial monolayers devoid of extracellular matrix to varying levels of tissue tension to study the orientation of cell divisions relative to the tissue plane. This analysis revealed that lowering tissue tension by compressing epithelial monolayers or by inhibiting myosin contractility increased the frequency of out-of-plane divisions. Reciprocally, increasing tissue tension by elevating cell contractility or by tissue stretching restored accurate in-plane cell divisions. Moreover, a characterization of the geometry of cells within these epithelia suggested that spindles can sense tissue tension through its impact on tension at subcellular surfaces, independently of their shape. Overall, these data suggest that accurate spindle orientation in the plane of the epithelium relies on a threshold level of tension at intercellular junctions.
540			_aCopyright © 2022 the Author(s). Published by PNAS.
540			_ahttps://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
546			_aen
690			_aBiological Sciences _92442
655	7		_aText _2local
786	0		_nProc Natl Acad Sci U S A
856	4	1	_uhttp://dx.doi.org/10.1073/pnas.2201600119 _zConnect to this object online.
999			_c425 _d425