000 02219 am a22002413u 4500
042 _adc
100 1 0 _aPlanelles-Herrero, Vicente Jose
_eauthor
_91489
700 1 0 _aBittleston, Alice
_eauthor
_91490
700 1 0 _aSeum, Carole
_eauthor
_91491
700 1 0 _aGaitan, Marcos Gonzalez
_eauthor
_91492
700 1 0 _aDerivery, Emmanuel
_eauthor
_91493
245 0 0 _aElongator stabilizes microtubules to control central spindle asymmetry and polarized trafficking of cell fate determinants
260 _c2022-10-27.
500 _a/pmc/articles/PMC7613801/
500 _a/pubmed/36302967
520 _aAsymmetric cell division gives rise to two daughter cells that inherit different determinants, thereby acquiring different fates. Polarized trafficking of endosomes containing fate determinants recently emerged as an evolutionary conserved feature of asymmetric cell division to enhance the robustness of asymmetric cell fate determination in flies, fish and mammals. In particular, polarized sorting of signalling endosomes by an asymmetric central spindle contributes to asymmetric cell division in Drosophila. However, how central spindle asymmetry arises remains elusive. Here, we identify a moonlighting function of the Elongator complex - an established protein acetylase and tRNA methylase involved in the fidelity of protein translation - as a key factor for central spindle asymmetry. Elongator controls spindle asymmetry by stabilizing microtubules differentially on the anterior side of the central spindle. Accordingly, lowering the activity of Elongator on the anterior side using nanobodies mistargets endosomes to the wrong cell. Molecularly, Elongator regulates microtubule dynamics independently of its acetylation and methylation enzymatic activities. Instead, Elongator directly binds to microtubules and increases their polymerisation speed while decreasing their catastrophe frequency. Our data establish a non-canonical role of Elongator at the core of cytoskeleton polarity and asymmetric signalling.
540 _a
546 _aen
690 _aArticle
655 7 _aText
_2local
786 0 _nNat Cell Biol
856 4 1 _uhttp://dx.doi.org/10.1038/s41556-022-01020-9
_zConnect to this object online.
999 _c813
_d813