| 000 | 02594 am a22003613u 4500 | ||
|---|---|---|---|
| 042 | _adc | ||
| 100 | 1 | 0 |
_aPirone, Daniele _eauthor _91645 |
| 700 | 1 | 0 |
_aLim, Joowon _eauthor _91646 |
| 700 | 1 | 0 |
_aMerola, Francesco _eauthor _91647 |
| 700 | 1 | 0 |
_aMiccio, Lisa _eauthor _91648 |
| 700 | 1 | 0 |
_aMugnano, Martina _eauthor _91649 |
| 700 | 1 | 0 |
_aBianco, Vittorio _eauthor _91650 |
| 700 | 1 | 0 |
_aCimmino, Flora _eauthor _91651 |
| 700 | 1 | 0 |
_aVisconte, Feliciano _eauthor _91652 |
| 700 | 1 | 0 |
_aMontella, Annalaura _eauthor _91653 |
| 700 | 1 | 0 |
_aCapasso, Mario _eauthor _91654 |
| 700 | 1 | 0 |
_aIolascon, Achille _eauthor _91655 |
| 700 | 1 | 0 |
_aMemmolo, Pasquale _eauthor _91656 |
| 700 | 1 | 0 |
_aPsaltis, Demetri _eauthor _91657 |
| 700 | 1 | 0 |
_aFerraro, Pietro _eauthor _91658 |
| 245 | 0 | 0 | _aStain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry |
| 260 | _c2022-12. | ||
| 500 | _a/pmc/articles/PMC7613862/ | ||
| 500 | _a/pubmed/36451849 | ||
| 520 | _aQuantitative Phase Imaging (QPI) has gained popularity in bioimaging because it can avoid the need for cell staining, which in some cases is difficult or impossible. However, as a result, QPI does not provide labelling of various specific intracellular structures. Here we show a novel computational segmentation method based on statistical inference that makes it possible for QPI techniques to identify the cell nucleus. We demonstrate the approach with refractive index tomograms of stain-free cells reconstructed through the tomographic phase microscopy in flow cytometry mode. In particular, by means of numerical simulations and two cancer cell lines, we demonstrate that the nucleus can be accurately distinguished within the stain-free tomograms. We show that our experimental results are consistent with confocal fluorescence microscopy (FM) data and microfluidic cytofluorimeter outputs. This is a significant step towards extracting specific three-dimensional intracellular structures directly from the phase-contrast data in a typical flow cytometry configuration. | ||
| 540 | _a | ||
| 540 | _ahttps://www.springernature.com/gp/open-research/policies/accepted-manuscript-termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms | ||
| 546 | _aen | ||
| 690 | _aArticle | ||
| 655 | 7 |
_aText _2local |
|
| 786 | 0 | _nNat Photonics | |
| 856 | 4 | 1 |
_uhttp://dx.doi.org/10.1038/s41566-022-01096-7 _zConnect to this object online. |
| 999 |
_c821 _d821 |
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