000 02594 am a22003613u 4500
042 _adc
100 1 0 _aPirone, Daniele
_eauthor
_91645
700 1 0 _aLim, Joowon
_eauthor
_91646
700 1 0 _aMerola, Francesco
_eauthor
_91647
700 1 0 _aMiccio, Lisa
_eauthor
_91648
700 1 0 _aMugnano, Martina
_eauthor
_91649
700 1 0 _aBianco, Vittorio
_eauthor
_91650
700 1 0 _aCimmino, Flora
_eauthor
_91651
700 1 0 _aVisconte, Feliciano
_eauthor
_91652
700 1 0 _aMontella, Annalaura
_eauthor
_91653
700 1 0 _aCapasso, Mario
_eauthor
_91654
700 1 0 _aIolascon, Achille
_eauthor
_91655
700 1 0 _aMemmolo, Pasquale
_eauthor
_91656
700 1 0 _aPsaltis, Demetri
_eauthor
_91657
700 1 0 _aFerraro, Pietro
_eauthor
_91658
245 0 0 _aStain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry
260 _c2022-12.
500 _a/pmc/articles/PMC7613862/
500 _a/pubmed/36451849
520 _aQuantitative Phase Imaging (QPI) has gained popularity in bioimaging because it can avoid the need for cell staining, which in some cases is difficult or impossible. However, as a result, QPI does not provide labelling of various specific intracellular structures. Here we show a novel computational segmentation method based on statistical inference that makes it possible for QPI techniques to identify the cell nucleus. We demonstrate the approach with refractive index tomograms of stain-free cells reconstructed through the tomographic phase microscopy in flow cytometry mode. In particular, by means of numerical simulations and two cancer cell lines, we demonstrate that the nucleus can be accurately distinguished within the stain-free tomograms. We show that our experimental results are consistent with confocal fluorescence microscopy (FM) data and microfluidic cytofluorimeter outputs. This is a significant step towards extracting specific three-dimensional intracellular structures directly from the phase-contrast data in a typical flow cytometry configuration.
540 _a
540 _ahttps://www.springernature.com/gp/open-research/policies/accepted-manuscript-termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms
546 _aen
690 _aArticle
655 7 _aText
_2local
786 0 _nNat Photonics
856 4 1 _uhttp://dx.doi.org/10.1038/s41566-022-01096-7
_zConnect to this object online.
999 _c821
_d821