000			02108 am a22003013u 4500
042			_adc
100	1	0	_aFiedler, Marc _eauthor _92461
700	1	0	_aFranco-Echevarría, Elsa _eauthor _92462
700	1	0	_aSchulten, Anna _eauthor _92463
700	1	0	_aNielsen, Mathias _eauthor _92464
700	1	0	_aRutherford, Trevor J. _eauthor _92465
700	1	0	_aYeates, Anna _eauthor _92466
700	1	0	_aAhsan, Bilal _eauthor _92467
700	1	0	_aDean, Caroline _eauthor _92468
700	1	0	_aBienz, Mariann _eauthor _92469
245	0	0	_aHead-to-tail polymerization by VEL proteins underpins cold-induced Polycomb silencing in flowering control
260			_bCell Press, _c2022-11-08.
500			_a/pmc/articles/PMC7614096/
500			_a/pubmed/36351412
520			_aTranscriptional silencing through the Polycomb silencing machinery utilizes a "read-write" mechanism involving histone tail modifications. However, nucleation of silencing and long-term stable transmission of the silenced state also requires P-olycomb Repressive Complex 2 (PRC2) accessory proteins, whose molecular role is poorly understood. The Arabidopsis VEL proteins are accessory proteins that interact with PRC2 to nucleate and propagate silencing at the FLOWERING LOCUS C (FLC) locus, enabling early flowering in spring. Here, we report that VEL proteins contain a domain related to an atypical four-helix bundle that engages in spontaneous concentration-dependent head-to-tail polymerization to assemble dynamic biomolecular condensates. Mutations blocking polymerization of this VEL domain prevent Polycomb silencing at FLC. Plant VEL proteins thus facilitate assembly of dynamic multivalent Polycomb complexes required for inheritance of the silenced state.
540			_aCrown Copyright © 2022.
540			_ahttps://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
546			_aen
690			_aArticle
655	7		_aText _2local
786	0		_nCell Rep
856	4	1	_uhttp://dx.doi.org/10.1016/j.celrep.2022.111607 _zConnect to this object online.
999			_c919 _d919