000 01638 am a22002653u 4500
042 _adc
100 1 0 _aZürcher, Jérôme F.
_eauthor
_92826
700 1 0 _aRobertson, Wesley E.
_eauthor
_92827
700 1 0 _aKappes, Tomás
_eauthor
_92828
700 1 0 _aPetris, Gianluca
_eauthor
_92829
700 1 0 _aElliott, Thomas S.
_eauthor
_92830
700 1 0 _aSalmond, George P. C.
_eauthor
_92831
700 1 0 _aChin, Jason W.
_eauthor
_92832
245 0 0 _aRefactored genetic codes enable bidirectional genetic isolation
260 _c2022-11-04.
500 _a/pmc/articles/PMC7614150/
500 _a/pubmed/36264827
520 _aThe near-universal genetic code defines the correspondence between codons in genes and amino acids in proteins. We refactored the structure of the genetic code in Escherichia coli and created orthogonal genetic codes that restrict the escape of synthetic genetic information into natural life. We developed orthogonal and mutually orthogonal horizontal gene transfer systems, which permit the transfer of genetic information between organisms that use the same genetic code but restrict the transfer of genetic information between organisms that use different genetic codes. Moreover, we showed that locking refactored codes into synthetic organisms completely blocks invasion by mobile genetic elements, which carry their own translation factors and successfully invade organisms with canonical and compressed genetic codes.
540 _a
546 _aen
690 _aArticle
655 7 _aText
_2local
786 0 _nScience
856 4 1 _uhttp://dx.doi.org/10.1126/science.add8943
_zConnect to this object online.
999 _c965
_d965